ZFIN Image: Zhao et al., 2023, Figure 6

Image

Figure Caption

Figure 6

Flow cytometric analysis of transgenic zebrafish, including Tg(lyz:DsRED2), Tg(rag2:DsRed), Tg(lck:EGFP), and Tg(mpeg1:EGFP). (A) The gating strategies in cytometric analysis for intestinal neutrophils (with red fluorescence labeled Lyz), immature lymphocytes (with red fluorescence labeled Rag2), mature T lymphocytes (with green fluorescence labeled Lck), and macrophages (with green fluorescence labeled Mpeg1) in zebrafish. Compare with wild type samples, all types of fluorescence labeled immune cells could be accurately sorted. (B) Comparing samples made by mechanical dissociation of whole intestine and blowing off mucosal cells from muscularis, the fluorescence labeled cells were significantly enriched. * represented p < 0.05, **** represented p < 0.0001.

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Front Immunol