Fig. 5

Stimulating the (auto)phagolysosomal axis increases the host survival against IA. (a, b and c) Survival curves of zebrafish embryos infected with A. fumigatus ?Ku80 and exposed via waterborne treatment to 10 nM of torin 1 (a), 10 ?M of AR-12 (b), 100 ?M or carbamazepine (c), or DMSO as a vehicle control at the same v:v. (a, b, and c) infections were performed together but showed in split graphs for visualization purposes. (d) Survival curve of dram1 mutants or non-mutant siblings infected with A. fumigatus ?Ku80. (e) Survival curve of zebrafish embryos overexpressing Dram1 after injection of 100 pg of dram1 mRNA or their control infected with A. fumigatus ?Ku80. (e) Confocal image demonstrating colocalization (arrowheads) of mCherry-Dram1 with Alexa Fluor? NHS 647-labeled A. fumigatus conidia (Af647) inside a phagocyte in the zebrafish hindbrain shortly after infection. All survival curves are representative of at least 3 independent biological replicates. The hazard ratio (HR) indicated is calculated vs. the control condition using the logrank method. Significance in the curve comparison is calculated using Log-rank (Mantel-Cox test): ns non-significant; * P ? 0.05; ** P ? 0.01; *** P ? 0.001; **** P ? 0.0001.