FIGURE

Fig. 7.

ID

ZDB-FIG-230420-149

Publication

Kugler et al., 2023 - GliaMorph: A modular image analysis toolkit to quantify Müller glial cell morphology

Other Figures

All Figure Page

Back to All Figure Page

Fig. 7.

Zonation and orientation measurements identify increased subcellular organization of MG cell processes during development. (A) Apicobasal intensity plotting using the zonationTool showed a statistically significant difference from 60 to 96 hpf in original (****P<0.0001), segmented (****P<0.0001) and skeletonized images (****P<0.0001) (60 hpf n=11, 72 hpf n=12, 96 hpf n=13; N=2 experimental repeats; Kruskal–Wallis test; mean). (B) Orientation measurement using Fourier transformation analysis delivered local orientation (yellow lines) in non-zero (black) regions. (C) Image as in B but colour-coded for orientation, showing that subcellular organization changed from a more vertical (1, yellow) to a more horizontal (0.2, blue) alignment. (D) Image order collapsed into 1D vectors for quantitative comparison (P=0.0049 for the comparison of the 60 and 96 hpf time points; data from two experimental repeats; Kruskal–Wallis test). a.u., arbitrary unit; ns, not significant.

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Development