Fig. 2
- ID
- ZDB-FIG-230405-3
- Publication
- Moore et al., 2022 - Microtubules are not required to generate a nascent axon in embryonic spinal neurons in vivo
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A. Image sequence showing the centrosome position of a neuron labelled with membrane and centrosome labels during apical process retraction. The centrosome is retracted close to the tip of the apical process. Images are maximum projections from confocal z-stacks. B. (i) Image sequence showing a neuron labelled with a membrane marker during apical retraction without any observable abscission event (n = 64/72 cells from 6 experiments). (ii) High-resolution images of boxed section in (i). Images are maximum projections from confocal z-stacks.< C. (i) Low magnification overview of the spinal cord of a cilium reporter line, Tg(actb2:arl13b-GFP) from a single confocal slice. (ii) High-resolution image sequence of boxed section in (i). One GFP-labelled cilium moves from apical surface towards the basal surface of the spinal cord (n = 13 cilia from two experiments). Images are maximum projections from confocal z-stacks. D. (i) Low magnification overview of the spinal cord of a Tg(actb2:arl13b-GFP) embryo labelled with centrin-RFP from a single confocal slice. (ii) Image sequence of boxed section in (i). A cilium and centrosome move together from apical surface towards the basal surface of the spinal cord (n = 2 cells from one experiment). Insets show high magnification of cilium-centrosome pair. Images are maximum projections from confocal z-stacks. E. Graph showing the length of cilium as it moves from apical surface (0%) to close to the basal surface (100%. Cilium length did not change; P > 0.05 for n = 6/7 cilia from two experiments; nonlinear regression). F. Distance between centrosome and cilium in Tg(actb2:arl13b-GFP) embryos fixed and processed for immunohistochemistry against GFP to label the cilium and ?-tubulin to label the centrosome. No difference was found in the distance between the two organelles when close to the apical surface or away from the midline (n = 50 cells per position from two experiments; P = 0.8279; midline mean = 0.4956, s.d. = 0.1125; away from midline mean = 0.5004, s.d. = 0.1077; Student's unpaired t-test). Bars show mean and standard deviation. Data information: All scale bars = 10 ?m. Source data are available online for this figure. |