FIGURE

Figure 1

ID

ZDB-FIG-230401-3

Publication

Pezzotta et al., 2023 - Combined Inhibition of Hedgehog and HDAC6: In Vitro and In Vivo Studies Reveal a New Role for Lysosomal Stress in Reducing Glioblastoma Cell Viability

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Figure 1

Hh and HDAC6 inhibition impaired U87-MG cell viability. (A) MTT assay of U87-MG cells treated for 48 h with vehicle (CT), 8 ?M of TubA (T8), 10 ?M of cyclo (C10) and 8 ?M of TubA + 10 ?M cyclo (T8C10). (B?E) Representative images of the head region of 3 dpf zebrafish embryos xenotransplanted with labeled U87-MG cells pretreated with (B) CT, (C) T8, (D) C10, and (E) T8C10. (F) Quantification of the tumor area at t1 (24 h postinjection, hpi) normalized to the tumor area at t0 (immediately after U87 injection). (G) Western blot analyses and quantification of pERK and pAKT protein expression levels following 48 h treatments with or without T8 and C10, alone or in combination. Data are expressed as the percentage of the control (A,G) or mean � standard deviation (F). CT?vehicle; TubA/T?tubastatin A; cyclo/C?cyclopamine. One-way ANOVA with Tukey post hoc correction. *** p < 0.001; ** p < 0.01; * p < 0.05; nonsignificant data are not shown. Scale bare indicates 100 ?m.

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

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