Figure 2

DICE-PPC-SRµCT at submicron resolution provides a detailed histological information. All images represent virtual thin sections from a 1mpf zebrafish scanned at 0.727µm voxel size. (A) Transverse view of the brain and optic nerves. (B) Longitudinal section through the spinal cord. (C) Transverse view of the eye, with the optic nerve and retinal layers visible. (D) Sagittal view of the crista ampullaris in the lateral semicircular canal. (E) Sagittal view of the heart. (F) Longitudinal view of the head region of the kidney. (G) Longitudinal view of the ovary. (H, I) Transverse and sagittal views of the pharyngeal teeth, with dental pulp visible. (J) Sagittal view of the two approximately perpendicular layers of hypaxial trunk musculature. (K) Sagittal view through the neurocranium (top) and hyomandibula (bottom), with visible chondrocyte lacunae and endochondral ossification of the hyomandibula. (L) Sagittal view through the dentary bone, using minimal projection with adjusted contrast to reveal osteocyte lacunae features in the bone. Inset image displays the same view but with comparable visualization settings to previous soft-tissue panels. (M) Sagittal section through the lapillus otolith, again with adjusted contrast to reveal the otolith microstructure showing growth rings. Inset image displays the same view but with comparable visualization settings to previous soft-tissue panels. (N) Section through the pectoral fin cartilaginous endoskeleton. ad, adipocytes; b, brain; ba, bulbus arteriosus; bc, body cavity; cu, cupula; dp, dental pulp; dr, distal radials; e, eye; em, epaxial muscle; gcl, ganglion cell layer; hme, hypaxial muscle (external); hmi, hypaxial muscle (internal); inl, inner nuclear layer; ipl, inner plexiform layer; lac, chondrocyte lacunae; na, neural arches; num, cell nuclei; oc, osteocytes; on, optic nerves; onl, outer nuclear layer; opl, outer plexiform layer; ot , optic tectum; pcl, photoreceptor cell layer; pr, proximal radials; r, rib; rbc, red blood cells; rpe, retinal pigmented epithelium; sv, sinus venosus. Scale bars, 100µm.