Figure 8

Juvenile nkx3.2 ^-/- zebrafish displays complete absence of the sinus perilymphaticus. (A, F) Lateral rendering of the sinus endolymphaticus (green) and sinus perilymphaticus (magenta) in the context of the occiput and Weberian apparatus (white, transparent) in 2mpf wild-type and nkx3.2 ^-/- zebrafish. (A?, F?) Anterodorsal renderings of the sinus endolymphaticus and sinus perilymphaticus (anterior to the left). (B-D, G?I) False coloured transverse, longitudinal, and sagittal virtual thin sections through the middle of the sinus endolymphaticus. Red asterisk in (I) indicates the blind posterior termination of the sinus impar. (E, J) False coloured transverse virtual thin sections through the atria of the sinus perilymphaticus. Red asterisks in (J) indicate the absence of the atria of the sinus perilymphaticus. Virtual thin section positions are indicated by labelled dotted lines in (A, A?). a, atrium; ast, asteriscus; cl, claustrum; dsu, ductus sacculo-utricularis; lag, lagena; sac, saccule; sc, scaphium; se, sinus endolymphaticus; sgt, sagitta; sp, sinus perilymphaticus; tc, transverse canal. Scale bars, 300µm.