FIGURE

Figure 2

ID

ZDB-FIG-230228-221

Publication

Prykhozhij et al., 2023 - Loss of calpain3b in Zebrafish, a Model of Limb-Girdle Muscular Dystrophy, Increases Susceptibility to Muscle Defects Due to Elevated Muscle Activity

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Figure 2

Expression pattern analysis of capn3b in wild-type and capn3b mutant zebrafish embryos. (A) Whole mount in situ hybridization with the capn3b probe, not including the deleted regions, was performed in wild-type, capn3b mut1/mut1, capn3b mut73/mut73, and capn3b rnaless/rnaless embryos at 28 and 52 hpf. (B) Whole mount in situ hybridization analysis with the capn3b and unc45b probes in wild-type and capn3b rnaless/rnaless embryos at 72 hpf. For each sample type, at least 40 embryos were stained. Representative images are shown in the figure (hpf = hours post-fertilization).

Expression Data

Genes:	capn3b unc45b
Fish:	mitfa^w2/w2; mpv17^a9/a9 mitfa^w2/w2; capn3b^hsi15/hsi15; mpv17^a9/a9 mitfa^w2/w2; capn3b^hsi16/hsi16; mpv17^a9/a9 mitfa^w2/w2; capn3b^hsi17/hsi17; mpv17^a9/a9
Anatomical Terms:	branchial muscle pharyngeal arch 3-7 post-vent region musculature system trunk musculature
Stage Range:	Prim-5 to Protruding-mouth

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	mitfa^w2/w2; capn3b^hsi15/hsi15; mpv17^a9/a9 mitfa^w2/w2; capn3b^hsi16/hsi16; mpv17^a9/a9 mitfa^w2/w2; capn3b^hsi17/hsi17; mpv17^a9/a9
Observed In:	pharyngeal arch 3-7 post-vent region musculature system trunk musculature
Stage Range:	Prim-5 to Protruding-mouth

Phenotype Detail

Acknowledgments

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