Figure 7

Loss- and gain-of lnc304 and lnc172 affected PGC development. (A) The fold change in endogenous lnc304 and lnc172 in embryos injected with lnc304 ASO gradients and lnc172 ASO gradients in comparison with embryo injected with control ASO (con ASO), respectively. (B?I) Representative images of the GFP-labelled PGCs in the embryos injected with con ASO (B), lnc304 ASO (C), lnc172 ASO (D), rfp (E), lnc304 (F), lnc172 (G), lnc304 + lnc304 ASO (H) and lnc172 + lnc172 ASO (I), respectively. Arrowheads indicate mislocalized PGCs. (J?M) The percentage of embryos with mislocalized PGC (J,L) and the number of mislocalized PGCs in each embryo (K,M) after injection of different RNAs and ASOs shown in (B?I) above. The columns represented for mean ± SD. Results were representative of more than three independent experiments in triplicate. ef1a and rpl13a were used as internal controls to normalize gene expression levels with 2^-??Ct method. * p < 0.05; ** p < 0.01; *** p < 0.001, **** p < 0.0001. Scale bar, 100 µm.