PUBLICATION
Two Novel lncRNAs Regulate Primordial Germ Cell Development in Zebrafish
- Authors
- Li, W., Liu, W., Mo, C., Yi, M., Gui, J.
- ID
- ZDB-PUB-230226-52
- Date
- 2023
- Source
- Cells 12(4): (Journal)
- Registered Authors
- Keywords
- cell migration, lncRNA, primordial germ cell, zebrafish
- MeSH Terms
-
- Animals
- Germ Cells
- In Situ Hybridization, Fluorescence
- Male
- RNA, Long Noncoding*
- Zebrafish*/genetics
- Zebrafish Proteins/genetics
- PubMed
- 36831339 Full text @ Cells
Citation
Li, W., Liu, W., Mo, C., Yi, M., Gui, J. (2023) Two Novel lncRNAs Regulate Primordial Germ Cell Development in Zebrafish. Cells. 12(4):.
Abstract
Long noncoding RNAs (lncRNAs) are regulatory transcripts in various biological processes. However, the role of lncRNAs in germline development remains poorly understood, especially for fish primordial germ cell (PGC) development. In this study, the lncRNA profile of zebrafish PGC was revealed by single cell RNA-sequencing and bioinformatic prediction. We established the regulation network of lncRNA-mRNA associated with PGC development, from which we identified three novel lncRNAs-lnc172, lnc196, and lnc304-highly expressing in PGCs and gonads. Fluorescent in situ hybridization indicated germline-specific localization of lnc196 and lnc304 in the cytoplasm and nucleus of spermatogonia, spermatocyte, and occyte, and they were co-localized with vasa in the cytoplasm of the spermatogonia. By contrast, lnc172 was localized in the cytoplasm of male germline, myoid cells and ovarian somatic cells. Loss- and gain-of-function experiments demonstrated that knockdown and PGC-specific overexpression of lnc304 as well as universal overexpression of lnc172 significantly disrupted PGC development. In summary, the present study revealed the lncRNA profile of zebrafish PGC and identified two novel lncRNAs associated with PGC development, providing new insights for understanding the regulatory mechanism of PGC development.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping