FIGURE

FIGURE 4

ID

ZDB-FIG-230228-108

Publication

Boyd et al., 2023 - Clcf1/Crlf1a-mediated signaling is neuroprotective and required for Müller glia proliferation in the light-damaged zebrafish retina

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FIGURE 4

Intravitreal injection of CLCF1/CRLF1 protects against photoreceptor cell death during light treatment. (A–D) Single z-plane confocal images from dark-adapted albino zebrafish that were light-treated for 24 h while being intravitreally injected with either PBS (A, C) or CLCF1/CRLF1 (B, D). TUNEL assay was performed to assess cell death, and nuclei were counterstained with DAPI. (E) Quantification showing significant decrease in the number of TUNEL-positive nuclei within the ONL of CLCF1/CRLF1-treated retinas. Student’s t-test, p = 0.0005, n ≥ 11. Mean ± SEM, ***p < 0.001. ONL, outer nuclear layer, INL, inner nuclear layer, GCL, ganglion cell layer. Scale bar represents 20 µm.

Expression Data

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Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Front Cell Dev Biol