FIGURE 4

Intravitreal injection of CLCF1/CRLF1 protects against photoreceptor cell death during light treatment. (A–D) Single z-plane confocal images from dark-adapted albino zebrafish that were light-treated for 24 h while being intravitreally injected with either PBS (A, C) or CLCF1/CRLF1 (B, D). TUNEL assay was performed to assess cell death, and nuclei were counterstained with DAPI. (E) Quantification showing significant decrease in the number of TUNEL-positive nuclei within the ONL of CLCF1/CRLF1-treated retinas. Student’s t-test, p = 0.0005, n ≥ 11. Mean ± SEM, ***p < 0.001. ONL, outer nuclear layer, INL, inner nuclear layer, GCL, ganglion cell layer. Scale bar represents 20 µm.