FIGURE

Fig. 3

ID

ZDB-FIG-230219-23

Publication

Hsu et al., 2022 - SIX1 reprograms myogenic transcription factors to maintain the rhabdomyosarcoma undifferentiated state

Other Figures

All Figure Page

Back to All Figure Page

Fig. 3

SIX1 KD inhibits human RMS growth and progression

(A and B) Bright-field images depicting the elongated cell morphology of SIX1 KD SMS-CTR and RD cells (A) and quantification of cell length (B). Statistical differences were calculated using one-way ANOVA followed by Dunnett?s multiple comparisons post hoc test.

(C) Tumor volumes, measured by caliper, over 12-weeks for Scramble and SIX1 KD SMS-CTR cells that were engrafted into the flanks of NSG mice. Data were fitted to a longitudinal mixed-effects model for statistical analysis of Scramble and SIX1 KD samples. Error bars represent mean � SD at each time point.

(D) Representative images of dissected Scramble or SIX1 KD xenografted tumors at 12 weeks.

(E) Final tumor weights in grams at the end of the study (n = 10 mice total). Statistical differences were calculated using one-way ANOVA followed by Dunnett?s multiple comparisons post hoc test.

(F) Representative H&E histology of dissected Scramble and SIX1 KD xenografted tumors (n = 5 tumors/KD).

(G) Representative pH3 immunostaining (brown) of dissected Scramble and SIX1 KD xenografted tumors. Dots in the graph represent the percentage of pH3+ cells per tumor section; pH3 staining was quantified over 2 sections per tumor. Statistical differences were calculated using one-way ANOVA followed by Dunnett?s multiple comparisons post hoc test.

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Cell Rep.