Fig. 3.

Combination of E-box and AP-1 motifs acts as the RRE. (A,B) Alignment of E-box (A) and AP-1 (B) motifs in the respective RREs. TRE, a consensus 12-O-tetradecanoylphorbol-13-acetate responsive element. The motifs with scores≥10.0 are shown. (C,D) Distribution of the E-box and AP-1 motifs in the previously reported RREs. Numbers, the Jaspar scores. Green boxes, regions homologous to TEs. (E) EGFP induction in the larval fin fold (left panels) and adult fin (right panels) of the Tg(6×E-0.7k:egfp) (n=3 Tg lines). (F) EGFP induction of Tg(6×E-0.7k:egfp) at different regeneration stages. zns5, osteoblasts. EGFP induction is seen in the distal WE and the fin ray mesenchymes that give rise to the blastema. (G) EGFP induction in the larval fin fold (left) and adult fin (right) of Tg(6×E-6×A:egfp) (n=2 Tg lines). (H) EGFP induction of Tg(6×E-6×A:egfp) at different regeneration stages. Arrowheads, amputation plane. *, fin ray; white arrows, EGFP in the basal epidermis; red arrows, EGFP in the fin ray mesenchyme and blastema. Scale bars: 50 µm (larvae), 500 µm (adult fin), and 100 µm (adult fin section). In all Tgs, EGFP expression was not detected in other body regions.