PUBLICATION
Splashed E-box and AP-1 motifs cooperatively drive regeneration response and shape regeneration abilities
- Authors
- Tamaki, T., Yoshida, T., Shibata, E., Nishihara, H., Ochi, H., Kawakami, A.
- ID
- ZDB-PUB-230114-2
- Date
- 2023
- Source
- Biology Open 12(2): (Journal)
- Registered Authors
- Kawakami, Atsushi, Ochi, Haruki
- Keywords
- Activator protein 1, E-box, Enhancer, Regeneration, Transgenic, Zebrafish
- MeSH Terms
-
- Animals
- Animals, Genetically Modified
- Conserved Sequence
- Promoter Regions, Genetic
- Transcription Factor AP-1*/metabolism
- Zebrafish*/metabolism
- PubMed
- 36636913 Full text @ Biol. Open
Citation
Tamaki, T., Yoshida, T., Shibata, E., Nishihara, H., Ochi, H., Kawakami, A. (2023) Splashed E-box and AP-1 motifs cooperatively drive regeneration response and shape regeneration abilities. Biology Open. 12(2):.
Abstract
Injury triggers a genetic program that induces gene expression for regeneration. Recent studies have identified regeneration-response enhancers (RREs); however, it remains unclear whether a common mechanism operates in these RREs. We identified three RREs from the zebrafish fn1b promoter by searching for conserved sequences within the surrounding genomic regions of regeneration-induced genes and performed a transgenic assay for regeneration response. Two regions contained in the transposons displayed RRE activity when combined with the -0.7 kb fn1b promoter. Another non-transposon element functioned as a stand-alone enhancer in combination with a minimum promoter. By searching for transcription factor-binding motifs and validation by transgenic assays, we revealed that the cooperation of E-box and activator protein 1 motifs is necessary and sufficient for regenerative response. Such RREs respond to variety of tissue injuries, including those in the zebrafish heart and Xenopus limb buds. Our findings suggest that the fidelity of regeneration response is ensured by the two signals evoked by tissue injuries. It is speculated that a large pool of potential enhancers in the genome has helped shape the regenerative capacities during evolution.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping