Figure 4

Effect of G-CSFR truncation mutation on adult hematopoiesis. (A–D). Adult blood cells from wild-type (wt/wt), heterozygous (wt/mdu26) and homozygous (mdu26/mdu26) mutant csf3r fish were subjected to Giemsa-staining (A–C), along with differential quantitation of the indicated blood cell populations for individual fish (D), with mean and SEM shown in red and level of statistical significance indicated (ns: not significant; n=4). Abbreviation: n: neutrophil. (E–P). Adult kidney cells from wild-type (wt/wt), heterozygous (wt/mdu27) and homozygous (mdu27/mdu27) mutant csf3r fish on a Tg(mpo::GFP) background were subjected to FACS analysis using SSC/FSC (E–G) and GFP fluorescence (I–K), along with quantitation of indicated cell populations (H) and GFP+ neutrophils (L), with sorted GFP+ cells subjected to Giemsa-staining (M–O), along with differential quantitation of relative differentiation (P). Panels (H, L, P) display results for individual fish, with mean and SEM shown in red and level of statistical significance indicated (*: p < 0.05, ns: not significant; n=4-9). im, immature; int; intermediate; m, mature.