Figure 3

A dMpv17 knockdown induced mitochondrial dysfunction in the larval CNS. (A) The relative mtDNA levels in the larval CNS extract of elav > GFP-IR, elav > dMpv17-IR⁴²⁶⁷¹, and elav > dMpv17-IR⁵⁶⁸⁸⁹ larvae were quantified. The relative levels of 16s rRNA (for mtDNA) and RpL32 (for nuclear DNA) were measured by qRT-PCR. **P < 0.01, statistical analysis was performed using one-way ANOVA followed by Dunnett?s multiple comparisons test vs elav > GFP-IR. n = 3. (B) The ATP levels in the larval CNS extracts of elav > GFP-IR, elav > dMpv17-IR⁴²⁶⁷¹, and elav > dMpv17-IR⁵⁶⁸⁸⁹ larvae were measured with Cell titer-Glo assay. *P < 0.05, statistical analysis was performed using one-way ANOVA followed by Dunnett?s multiple comparisons test vs elav > GFP-IR. n = 5. (C) The lactate level in the larval CNS extract of elav > GFP-IR, elav > dMpv17-IR⁴²⁶⁷¹, and elav > dMpv17-IR⁵⁶⁸⁸⁹ larvae were measured with the Lactate-Glo assay. **P < 0.01, *P < 0.05, statistical analysis was performed using one-way ANOVA followed by Dunnett?s multiple comparisons test vs elav > GFP-IR. n = 3. (D) Pyruvate levels in the CNS extracts of elav > GFP-IR, elav > dMpv17-IR⁴²⁶⁷¹, and elav > dMpv17-IR⁵⁶⁸⁸⁹ larvae were measured with a pyruvate assay kit. **P < 0.01, *P < 0.05, statistical analysis was performed using one-way ANOVA followed by Dunnett?s multiple comparisons test vs elav > GFP-IR. n = 3. (E) Third instar larval CNSs were stained with CM-H₂DCFCA for the detection of ROS. Representative ROS images of the CNS of elav > GFP-IR, elav > dMpv17-IR⁴²⁶⁷¹, and elav > dMpv17-IR⁵⁶⁸⁸⁹ are shown. Scale bar, 100 ?m. (F) The fluorescence intensity in the CNS area enclosed by the white line was quantified. **P < 0.01, statistical analysis was performed using Student?s t-test vs elav > GFP-IR. n = 5.