FIGURE

Fig 4

ID

ZDB-FIG-221230-22

Publication

Chen et al., 2022 - Loss of growth differentiation factor 9 causes an arrest of early folliculogenesis in zebrafish-A novel insight into its action mechanism

Other Figures

All Figure Page

Back to All Figure Page

Fig 4

Prevention of sex reversal of gdf9 mutant by dmrt1 mutation.

(A) Gonadal histology at 60 and 120 dpf. Ovary could form in the control (dmrt1+/-;gdf9+/-), single mutants (dmrt1-/- and gdf9-/-) and double mutant (dmrt1-/-;gdf9-/-) as seen at 60 dpf. The gdf9-/- single mutant became all males at 120 dpf (16/16 fish examined in total) whereas most individuals of the double mutant were females (8/14 examined), similar to that in the control (9/15). The numbers shown in the photos indicate the total number of fish examined (lower) and the number of female fish exhibiting similar phenotype to that shown (upper). (B) Change of sex ratio during 60–190 dpf. The offspring from one crossing (dmrt1+/-;gdf9+/- female X dmrt1+/-;gdf9+/- male) were sampled at different times from 60 to 190 dpf for genotyping and sex identification by histology. The gdf9-/- fish showed a male-biased sex ratio at 60 dpf, and it became all-male from 120 dpf due to sex reversal. Double mutation with dmrt1 rescued the sex ratio to the control level with even higher female ratios at 150 and 190 dpf. PG, primary growth; PV, pre-vitellogenic; FG, full-grown; SC, spermatocytes; SZ, spermatozoa.

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ PLoS Genet.