ZFIN Image: Chen et al., 2022, Fig 4

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Figure Caption

Fig 4

Prevention of sex reversal of gdf9 mutant by dmrt1 mutation.

(A) Gonadal histology at 60 and 120 dpf. Ovary could form in the control (dmrt1+/-;gdf9+/-), single mutants (dmrt1-/- and gdf9-/-) and double mutant (dmrt1-/-;gdf9-/-) as seen at 60 dpf. The gdf9-/- single mutant became all males at 120 dpf (16/16 fish examined in total) whereas most individuals of the double mutant were females (8/14 examined), similar to that in the control (9/15). The numbers shown in the photos indicate the total number of fish examined (lower) and the number of female fish exhibiting similar phenotype to that shown (upper). (B) Change of sex ratio during 60–190 dpf. The offspring from one crossing (dmrt1+/-;gdf9+/- female X dmrt1+/-;gdf9+/- male) were sampled at different times from 60 to 190 dpf for genotyping and sex identification by histology. The gdf9-/- fish showed a male-biased sex ratio at 60 dpf, and it became all-male from 120 dpf due to sex reversal. Double mutation with dmrt1 rescued the sex ratio to the control level with even higher female ratios at 150 and 190 dpf. PG, primary growth; PV, pre-vitellogenic; FG, full-grown; SC, spermatocytes; SZ, spermatozoa.

Acknowledgments

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