Fig. 5

a Imaging of VGNs (top). VGNs in the rostral division of VG in a 5 dpf Tg(myo6b:jGCaMP7f; myo6b:tdTomato) larva imaged from ventral (bottom). Red channel is shown. Scale bar: 10 ?m. The same orientation applies to (b)?(e), (g), and (h). b VGN responses to 90° static tilt (see Supplementary Movie ⁵). Tilt time course (top). Sequential images showing mean ?R/R₀ responses during the time period ?i? to ?vi? (bottom). Maximum intensity projection of six optical slices with 5 ?m intervals. Single trial at each slice. Dashed lines: VG contours. Scale bar: 10 ?m. The same ganglion is shown in (c), (d), (e), and (g). Response selectivity for tilt direction in images of maximum intensity projection (c) and different depth (d). Tail-down response (?v? in [b]) subtracted from nose-down response (?ii? in [b]) (top). Medial-down response (?v? in [b]) subtracted from lateral-down response (?ii? in [b]) (bottom). Dashed lines: VG contours. Scale bar: 10 ?m. Average of 6 ganglia ([c], right). e Tilt responses per VGN. VGN location (top, 6 optical slices overlaid). Tilt time course (middle). ?R/R₀ responses (bottom). f Mean ?R/R₀ responses across VGNs in each preference group in a single VG (top). Number of cells is reported in the Supplementary Fig. ⁴ legend. Mean responses of each group (bottom, 6 VG for pitch; 3 left VG for roll; 3 right VG are shown in Supplementary Fig. ^4e). SEM is shown in gray. Tilt time course is the same as in (e). Scale bar: 50% ?R/R₀. g VGN response vectors. Orientation and length of arrows indicate preferred direction and ?R/R₀ amplitude, respectively. Responses with amplitude larger than 10% ?R/R₀ are shown. Gray: VGN contours. Magenta scale bar: 50% ?R/R₀. Black scale bar: 10 ?m. (right-end panel) All response vectors aligned together. Black bar: mean response vector. Magenta scale bar: 50% ?R/R₀. h Summary of VGN response vectors from 6 ganglia. Mean response vectors per grid (see ?Methods?). Arrows and gray dots show mean response vectors with amplitude larger or not larger than 10% ?R/R₀, respectively. Colors and scale bars are the same as in (g). Source data are provided as a Source Data file.