Fig. 5

Fig. 5. A phylogenetic tree of the protein sequences of M14A carboxypeptidases CPA1, CPA2, CPA3, CPA4, CPA5, CPA6, CPO, CPD and CPM. The tree was constructed using MrBayes with the MCMC method and the sequences were obtained from NCBI tblastn by homology search. Phylogenetic tree of carboxypeptidase relationships based on Bayesian inference with the MrBayes program of CPs genes. Numbers on nodes indicate posterior probabilities. The branches representing the different CPs are depicted in the same colors as in the loci Fig. 1, Fig. 2, Fig. 3 and with CPA1 in brown, CPA2 in red, CPA3 in dark blue, CPA4 in purple, CPA5 in beige, CPA6 dark green, CPO in orange, CPB1 in light blue and CPB2 in light green. Noteworthy is the inclusion of the related M14B CPs, CPD and CPM in the analysis, belonging to the related M14B subfamily, appearing in black in the upper branch of the tree, as an outgroup to obtain a more robust tree.