FIGURE

Fig. 4

ID

ZDB-FIG-221212-20

Publication

Motta et al., 2021 - SPRED2 loss-of-function causes a recessive Noonan syndrome-like phenotype

Other Figures

All Figure Page

Back to All Figure Page

Fig. 4

p.Leu100Pro results in a defective SPRED2 binding to neurofibromin

(A) Subcellular localization of transiently expressed V5-tagged GRD and Xpress-tagged WT or SPRED2^Leu100Pro (L100P) proteins in COS-1 cells after stimulation with EGF (30 ng/mL, 5 min) revealed by confocal microscopy analysis. Cells were stained using anti-Xpress IgG1 and anti-V5 IgG2a monoclonal antibodies and revealed by goat anti-mouse IgG1 Alexa Fluor 647 (pseudocolored in green) and goat anti-mouse IgG2a Alexa Fluor 594 (red) secondary antibodies, respectively. Nuclei are visualized by DAPI staining (blue). Scale bar, 10 μm.

(B) Co-immunoprecipitation assays. Lysates from HEK293T cells transiently transfected to express WT or variant Xpress-tagged SPRED2 protein were serum-starved, stimulated with EGF (30 ng/mL), and immunoprecipitated with an anti-Xpress antibody and assayed by western blotting using the indicated antibodies.

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Am. J. Hum. Genet.