FIGURE

Fig. 5

ID

ZDB-FIG-221211-303

Publication

Morales-Curiel et al., 2022 - Volumetric imaging of fast cellular dynamics with deep learning enhanced bioluminescence microscopy

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Fig. 5

Seamless denoising and segmentation of bioluminescent zebrafish embryos and mouse embryonic stem cells.

a Laser scanning confocal fluorescence image of a 4 h post-fertilization (hpf) zebrafish embryo expressing membrane-bound GPI-GFP taken on a Leica SP5. The red box indicates the close-up below. Scale bar = 50 ?m. b Unprocessed bioluminescence image of a 4 hpf zebrafish embryo expressing GPI-GFP targeted to the plasma membrane. The red square indicates the high magnification close-up below. Scale bar = 100 ?m. c The same bioluminescent signal of the zebrafish embryo was restored using a pretrained CARE pipeline optimized for epithelial monolayers. The bottom picture shows the segmented bioluminescent image. No segmentation was possible on the raw image. Scale bar = 20 ?m. d Brightfield (i) and fluorescence image (ii) of a spheroid of mouse embryonic stem cells. e Unprocessed raw (i) and denoised (ii) bioluminescence image of similar spheroids. Scale bar = 75 ?m. f Segmented nuclei (i) after denoising, overlayed with their individual tracks throughout the timelapse (ii). Scale bar = 35 ?m.

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

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