PUBLICATION
Volumetric imaging of fast cellular dynamics with deep learning enhanced bioluminescence microscopy
- Authors
- Morales-Curiel, L.F., Gonzalez, A.C., Castro-Olvera, G., Lin, L.L., El-Quessny, M., Porta-de-la-Riva, M., Severino, J., Morera, L.B., Venturini, V., Ruprecht, V., Ramallo, D., Loza-Alvarez, P., Krieg, M.
- ID
- ZDB-PUB-221204-9
- Date
- 2022
- Source
- Communications biology 5: 13301330 (Journal)
- Registered Authors
- Loza-Alvarez, Pablo
- Keywords
- none
- MeSH Terms
-
- Animals
- Caenorhabditis elegans
- Cell Nucleus
- Cytoplasm
- Deep Learning*
- Mice
- Microscopy, Fluorescence
- Zebrafish
- PubMed
- 36463346 Full text @ Commun Biol
Citation
Morales-Curiel, L.F., Gonzalez, A.C., Castro-Olvera, G., Lin, L.L., El-Quessny, M., Porta-de-la-Riva, M., Severino, J., Morera, L.B., Venturini, V., Ruprecht, V., Ramallo, D., Loza-Alvarez, P., Krieg, M. (2022) Volumetric imaging of fast cellular dynamics with deep learning enhanced bioluminescence microscopy. Communications biology. 5:13301330.
Abstract
Bioluminescence microscopy is an appealing alternative to fluorescence microscopy, because it does not depend on external illumination, and consequently does neither produce spurious background autofluorescence, nor perturb intrinsically photosensitive processes in living cells and animals. The low photon emission of known luciferases, however, demands long exposure times that are prohibitive for imaging fast biological dynamics. To increase the versatility of bioluminescence microscopy, we present an improved low-light microscope in combination with deep learning methods to image extremely photon-starved samples enabling subsecond exposures for timelapse and volumetric imaging. We apply our method to image subcellular dynamics in mouse embryonic stem cells, epithelial morphology during zebrafish development, and DAF-16 FoxO transcription factor shuttling from the cytoplasm to the nucleus under external stress. Finally, we concatenate neural networks for denoising and light-field deconvolution to resolve intracellular calcium dynamics in three dimensions of freely moving Caenorhabditis elegans.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping