Figure 4

p66Shc hypomorphic allele embryo diameter and adult zebrafish mass. (A) Embryo diameter (top left panel) and embryo diameter frequency distribution of different parental crosses. p66Shc hypomorphic allele heterozygote females produce embryos with a bimodal size distribution that can be separated under a microscope. Size differences were significant and not observed when female WT (F GFP ^+/+) fish were bred with male WT (M GFP ^+/+) or male p66Shc^+/− (M hypomorphic allele (HA) ^+/−). Parental genotypes are indicated by color and above their corresponding histograms. N = 50, **** = p < 0.0001, mean ± SD. (B) Whole fish weight differences (left panel) by gender and genotype. Males had a smaller mass decrease than females. Whole fish and heart mass differences (right panel), not gender stratified. N = 16 (hearts) or ≥ 36 (whole fish), * = p < 0.05, mean ± SD. Individual data points omitted for clarity.