FIGURE

Fig. 2

ID

ZDB-FIG-221123-17

Publication

Fowler et al., 2021 - Protein fucosylation is required for Notch dependent vascular integrity in zebrafish

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Fig. 2

Salvage pathway genes contribute to vascular integrity. The ?fcsk ?gene is expressed ubiquitously in the head of wildtype 48 hpf embryos (A) compared to the mild background staining in the wildtype control embryos using a sense probe (B). The ?fpgt ?gene was expressed ubiquitously in the head of wildtype 48 hpf embryos (C) compared to the mild background staining in the wildtype control embryos using a sense probe (D). The recorded cerebral hemorrhaging rates of embryo clutches derived from ?gmds ?heterozygous matings and control wildtype crosses with or without ?fcsk ?morpholino (E), showing that the ?fcsk ?morpholino increase hemorrhage rates. (F), results of rescue experiments using GDP-fucose and L-fucose, demonstrating that both conditions can rescue hemorrhage rate. Statistical significance was calculated using a chi-squared test with Yate's correction. Error bars represent standard error of the mean hemorrhage rate from three independent experiments.

Expression Data

Genes:	fcsk fpgt
Fish:	AB
Anatomical Term:	head
Stage:	Long-pec

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	gmds^nfl2/nfl2 (AB) AB + MO1-fcsk + MO4-tp53 gmds^nfl2/nfl2 + MO1-fcsk + MO4-tp53 (AB)
Conditions:	chemical treatment by injection: GDP-L-fucose chemical treatment by injection: L-fucose
Knockdown Reagents:	MO1-fcsk MO4-tp53
Observed In:	brain vasculature
Stage Range:	Long-pec to Protruding-mouth

Phenotype Detail

Acknowledgments

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Reprinted from Developmental Biology, 480, Fowler, G., French, D., Rose, A., Squires, P., Anecito da Silva, C., Ohata, S., Okamoto, H., French, C.R., Protein fucosylation is required for Notch dependent vascular integrity in zebrafish, 62-68, Copyright (2021) with permission from Elsevier. Full text @ Dev. Biol.