FIGURE

Fig. 3

ID

ZDB-FIG-221118-25

Publication

Wang et al., 2022 - A conserved megaprotein-based molecular bridge critical for lipid trafficking and cold resilience

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Fig. 3

a Representative confocal fluorescence images showing PIP3-binding Akt-PH::GFP reporters in wild type (apical intestinal membrane, arrow; basolateral membrane, arrow head) and lpd-3(ok2138) mutants at both low and high magnifications. b Representative confocal fluorescence images showing the actin reporter act-5p::act-5::GFP in wild type (apical intestinal membrane, arrow) and lpd-3(ok2138) mutants. c Representative bright-field and epifluorescence images showing activation of fat-7::GFP by RNAi against sams-1. d Representative bright-field and epifluorescence images showing activation of fat-7::GFP by RNAi against sams-1 in wild type but not lpd-3(ok2138) mutants (arrow). e Representative epifluorescence images showing activation of the hsp-4p::GFP ER stress reporter by RNAi against sams-1 in wild type but not lpd-3(ok2138) mutants (arrow). f Representative confocal fluorescence images showing reduced abundance of nuclear sbp-1p::sbp-1::GFP by RNAi against sbp-1 or lpd-3. g Representative epifluorescence images showing no apparent changes of sbp-1p::GFP by RNAi against sbp-1 or lpd-3. h Representative epifluorescence images showing markedly increased membrane permeability for fluorescein in lpd-3(ok2138) mutants. i Table summary of reporter phenotypes of indicated genotypes or conditions. Scale bars, 50 µm.

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Nat. Commun.