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Fig. 3 a Representative confocal fluorescence images showing PIP3-binding Akt-PH::GFP reporters in wild type (apical intestinal membrane, arrow; basolateral membrane, arrow head) and lpd-3(ok2138) mutants at both low and high magnifications. b Representative confocal fluorescence images showing the actin reporter act-5p::act-5::GFP in wild type (apical intestinal membrane, arrow) and lpd-3(ok2138) mutants. c Representative bright-field and epifluorescence images showing activation of fat-7::GFP by RNAi against sams-1. d Representative bright-field and epifluorescence images showing activation of fat-7::GFP by RNAi against sams-1 in wild type but not lpd-3(ok2138) mutants (arrow). e Representative epifluorescence images showing activation of the hsp-4p::GFP ER stress reporter by RNAi against sams-1 in wild type but not lpd-3(ok2138) mutants (arrow). f Representative confocal fluorescence images showing reduced abundance of nuclear sbp-1p::sbp-1::GFP by RNAi against sbp-1 or lpd-3. g Representative epifluorescence images showing no apparent changes of sbp-1p::GFP by RNAi against sbp-1 or lpd-3. h Representative epifluorescence images showing markedly increased membrane permeability for fluorescein in lpd-3(ok2138) mutants. i Table summary of reporter phenotypes of indicated genotypes or conditions. Scale bars, 50 µm.