Figure 5

The expression of cell proliferation markers and histopathological features from H&E stain in [tert] and [tert x p53^−/−] transgenic fish. The expression of proliferation marker in [tert] fish at 15 dpf and 30 dpf (a) ccne1, (b) cdk1, and (c) cdk2, and in [tert x p53^−/−] fish at 15 dpf and 30 dpf (d) ccne1, (e) cdk1, and (f) cdk2. *: p ≦ 0.05; **: p ≦ 0.01; ***: p ≦ 0.001. (g) The representative images of H&E stain of WT, [tert], and [tert x p53^−/−] fish at 30 dpf. The blue circle indicates the mitotic features, the green square points out the trinucleated hepatocytes, the red square denotes the karyomegalic cells. The scale bar is 20 µm in length. The quantification of histopathological features from H&E stain: (h) mitotic figures, (i) trinucleated hepatocytes, (j) karyomegalic cells are increased in WT, [tert], and [tert x p53^−/−] fish at 30 dpf. We performed three replicates, and for each qPCR, there were triplicates for each sample. Ten embryos were included in each experiment. One-way ANOVA analysis followed by multiple analysis was used to identify the statistical significance between groups. *: p ≦ 0.05; **: p ≦ 0.01; ***: p ≦ 0.001; ****: p ≦ 0.0001; ns: no-significance.