FIGURE

Figure 3

ID

ZDB-FIG-221029-24

Publication

Davison et al., 2022 - Zebrafish Slit2 and Slit3 Act Together to Regulate Retinal Axon Crossing at the Midline

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Figure 3

Retinal axons from slit2 + slit3 crispants innervate the ipsilateral optic tectum. Retinotopic anterograde RGC axon DiI labeling in atoh7:Gap43-EGFP (atoh7:GFP) transgenic larvae. (A?D). Horizontal maximum intensity z-projections of the cephalic region of 5 dpf larvae, where both the contralateral and ipsilateral tecta can be observed (dorsal view). Non-injected wild-type (A), slit2 crispant (B), slit3 crispant (C) and slit2 + slit3 crispant (D) larvae are shown. DiI-labeled axons are observed in the ipsilateral tectum of slit2 + slit3 crispants (arrows), and can be better visualized in the rotated view in d?. DiI-labeled axons can also be observed crossing the midline at a site slightly posterior to the optic tract (arrowheads). Scale bars: (A?D), 30 ?m; (d?), 20 ?m. See Video S3.

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

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