ZFIN Image: Davison et al., 2022, Figure 3

Image

Figure Caption

Figure 3

Retinal axons from slit2 + slit3 crispants innervate the ipsilateral optic tectum. Retinotopic anterograde RGC axon DiI labeling in atoh7:Gap43-EGFP (atoh7:GFP) transgenic larvae. (A?D). Horizontal maximum intensity z-projections of the cephalic region of 5 dpf larvae, where both the contralateral and ipsilateral tecta can be observed (dorsal view). Non-injected wild-type (A), slit2 crispant (B), slit3 crispant (C) and slit2 + slit3 crispant (D) larvae are shown. DiI-labeled axons are observed in the ipsilateral tectum of slit2 + slit3 crispants (arrows), and can be better visualized in the rotated view in d?. DiI-labeled axons can also be observed crossing the midline at a site slightly posterior to the optic tract (arrowheads). Scale bars: (A?D), 30 ?m; (d?), 20 ?m. See Video S3.

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ J Dev Biol