FIGURE

Figure 3.

ID

ZDB-FIG-221018-18

Publication

Deng et al., 2022 - Zbtb14 regulates monocyte and macrophage development through inhibiting pu.1 expression in zebrafish

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Figure 3.

Overproliferation of macrophage progenitor is the main cause of macrophage lineage expansion in zbtb14 mutants.

(A–C’, E–G’) pH3 and transferase dUTP nick end labeling (TUNEL) assays in zbtb14^-/-//Tg(mpeg1.1:eGFP) and Tg(mpeg1:EGFP) control larvae. Triangles indicate positive signals. (D, H) Statistical result for A–C’ and E–G’. Data shown are the means ± standard error of the mean (SEM) of at least 15 larvae. ns: not statistically significant, **p<0.01. (I) Classification of zebrafish monocytes/macrophages is based on their morphology. (J) Quantification of mpeg1.1 ⁺ cells at each differentiation stage. Sorted macrophage lineage cells from 10-month-old zbtb14^-/-//Tg(mpeg1.1:eGFP) and Tg(mpeg1.1:eGFP) larvae were subjected to May-Grünwald-Giemsa staining and separated into immature, intermediate, and mature groups according to their morphology. Scale bar: 10 µm. Error bars represent mean ± SEM of three independent experiments. *p<0.05 (Student’s t-test).

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

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