PUBLICATION
Zbtb14 regulates monocyte and macrophage development through inhibiting pu.1 expression in zebrafish
- Authors
- Deng, Y., Wang, H., Liu, X., Yuan, H., Xu, J., de Thé, H., Zhou, J., Zhu, J.
- ID
- ZDB-PUB-221008-4
- Date
- 2022
- Source
- eLIFE 11: (Journal)
- Registered Authors
- Zhu, Jun
- Keywords
- developmental biology, zebrafish
- MeSH Terms
-
- Animals
- Cell Differentiation/genetics
- Lysine/metabolism
- Macrophages/metabolism
- Monocytes*/metabolism
- Phenylalanine/metabolism
- Serine/metabolism
- Transcription Factors/genetics
- Transcription Factors/metabolism
- Zebrafish*/genetics
- PubMed
- 36205309 Full text @ Elife
Citation
Deng, Y., Wang, H., Liu, X., Yuan, H., Xu, J., de Thé, H., Zhou, J., Zhu, J. (2022) Zbtb14 regulates monocyte and macrophage development through inhibiting pu.1 expression in zebrafish. eLIFE. 11:.
Abstract
Macrophages and their precursor cells, monocytes, are the first line of defense of the body against foreign pathogens and tissue damage. Although the origins of macrophages are diverse, some common transcription factors (such as PU.1) are required to ensure proper development of monocytes/macrophages. Here we report that the deficiency of zbtb14, a transcription repressor gene belonging to ZBTB family, leads to an aberrant expansion of monocyte/macrophage population in zebrafish. Mechanistically, Zbtb14 functions as a negative regulator of pu.1, and SUMOylation on a conserved lysine is essential for the repression activity of Zbtb14. Moreover, a serine to phenylalanine mutation found in an acute myeloid leukemia (AML) patient could target ZBTB14 protein to autophagic degradation. Hence, ZBTB14 is a newly identified gene implicated in both normal and malignant myelopoiesis.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping