Gene ontology (GO) annotations retrieved from plasma proteins in the zebrafish λ-CGN-induced inflammatory reaction after 4h. (A) The forty-two differentially expressed genes were involved in biological processes, mainly in metabolic process (13%) and transport (7%) as the top terms. (B) Major protein-protein interaction network among hub protein clusters in the blood plasma of λ-CGN-induced fish. The stronger associations are represented with thicker lines. (C) Functional pathway network showing the distribution of pathways associated with the complement system and blood coagulation cascade identified through the functional enrichment analysis of λ-CGN-treated fish. The color depth of nodes refers to the corrected p-value of the ontologies represented: (purple, immunoglobulins; green, apolipoproteins; orange, complement; blue, fibrinogen). Hierarchical clustering of hub protein-protein interaction networks functional enrichment analysis was constructed using STRING (https://string-db.org). The associations between the twenty-seven proteins of this signaling pathway were predicted using the KEGG tool. Names and taxonomy retrieved from UniProtKB (See Table 1 for accession numbers): Keratin 5 (Krt5); Keratin 8 (Krt8); Cofilin 1 (Cfl1); Myosin heavy chain 4 (Myhc4); Actin (Actb1); Retinol binding protein 4 (Rbp4); Alpha-2-macroglobulin-like protein (A2ml); Alpha-2-macroglobulin-like 1 (Si:dkey-105h12.2); Alpha-2-macroglobulin (Sb:cb37); Apolipoprotein Eb (Apoeb); Apolipoprotein A-IV b,2 (Apoa4b.2); Apolipoprotein A-IV b,1 (Apoa4b.1); Phosphoglycerate mutase (Pgam2); Phosphopyruvate hydratase (Eno1); Fructose-bisphosphate aldolase, b (Aldoab); Fructose-bisphosphate aldolase, a (Aldoaa); Kininogen 1 (Kng1); Fibrinogen gamma chain precursor (Fgg); Antithrombin-III precursor (Serpinc1); Prothrombin (F2); Complement C3a, tandem 2 (C3a.2); Hemopexin (Zgc:152945-001); Complement factor B (Cfb); Plasminogen (Plg); and, Chymotrypsinogen B,1 (Ctrb.1).
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