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Fig. 1

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ZDB-FIG-221014-1

Publication

Yu et al., 2021 - Mechanism of gating and partial agonist action in the glycine receptor

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Fig. 1

Single channel electrophysiology and cryo-EM analysis of the glycine complex.

A. Single-channel recording of GlyR openings in the presence of 10 mM glycine.

B. Fractions of GlyR particles in the desensitized, open and expanded/super-open states in nanodiscs, SMA, or detergent micelles. Error bars represent SEM, as described in the Methods.

C. Plots of pore radius for glycine-bound states. The C? position of Arg 268 (M2 0?) is set to 0.

D-F. Cryo-EM density maps. The partially transparent surface represents lipid-SMA density.

G-I. Ion permeation pathways where M2 helices of two subunits are shown in ribbon and the side chains of pore-lining residues are shown in sticks, calculated by the program HOLE (radii coloring: red <1.8 �, green 1.8?3.3 �, and blue > 3.3 �).

See also Figures S1?S2, Tables S1?S2, S5, S7 and Data S1

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

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Reprinted from Cell, 184, Yu, J., Zhu, H., Lape, R., Greiner, T., Du, J., L�, W., Sivilotti, L., Gouaux, E., Mechanism of gating and partial agonist action in the glycine receptor, 957-968.e21, Copyright (2021) with permission from Elsevier. Full text @ Cell