FIGURE 3

Antimicrobial activity in WT and cftr-LOF zebrafish embryos of the combination treatment with phosphatidylserine/phosphatidic acid (PS/PA) liposome in prophylactic administration and phage therapy. (A) Schematic representation of PS/PA liposome prophylactic treatment followed by systemic bacterial infection and CKΦ-administration. Zebrafish embryos were treated with PS/PA liposome at 28 hpf, then systemically infected with PAO1-GFP at 48 hpf, treated with CKΦ 3 h later, and analyzed for bacterial burden at 8 or 20 h post infection. (B,C) Bacterial load (relative CFU/embryo) in systemically infected wild-type (WT) (B), and cftr-LOF embryos (C), control (ctrl), PS/PA liposome, CKΦ and PS/PA liposome + CKΦ treated. Statistical significance was assessed by One-way ANOVA followed by Tukey’s post hoc test: ***p < 0.001; *p < 0.05; ns, not significant. Data resulted from three independent experiments and results are presented as mean ± SEM.