FIGURE

Fig. 2

ID

ZDB-FIG-220922-54

Publication

Niescierowicz et al., 2022 - Adar-mediated A-to-I editing is required for embryonic patterning and innate immune response regulation in zebrafish

Other Figures

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Fig. 2

a Wild-type (b, c) Adar MO-injected embryos develop abnormal phenotype in the posterior part with disturbed body axis, shortened tail and crooked, disorganized notochord. d MO phenotype can be fully rescued with wild-type mRNA injection. e Mutant adar mRNA E1030A with inactivated editing domain could not rescue the malformed phenotype. f, g Phenotype defects caused by adar mRNA overexpression. The anterior defects, including cyclopia and head malformations are mRNA dose dependent. Inset marked by red boxes denotes overlaid identical image taken at different focal plane. Observation was performed on embryos from four independent adult pairs with similar results. h, i Injection statistics of Adar MO, rescue, and mRNA overexpression. Source data are provided in the Source data file.

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	AB + MO3-adar
Knockdown Reagent:	MO3-adar
Observed In:	head notochord post-vent region
Stage:	Prim-5

Phenotype Detail

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Nat. Commun.