FIGURE

Fig. 3

ID

ZDB-FIG-220920-60

Publication

Tyrkalska et al., 2022 - Differential proinflammatory activities of Spike proteins of SARS-CoV-2 variants of concern

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Fig. 3

Fig. 3. Ace2 deficiency exacerbates the proinflammatory activity of S1WT in zebrafish.

One-cell-stage zebrafish eggs of Tg(mpx:eGFP) (A and H), Tg(mfap4:mCherry) (B), Tg(nfkb:eGFP) (C), and WT (D to G) were microinjected with control or ace2 crRNA-Cas9 complexes. At 2 dpf, recombinant S1WT, flagellin, or vehicle (−) was injected alone or in combination with Ang (1-7) in the hindbrain ventricle of control and Ace2-deficient larvae. Neutrophil (A and H) and macrophage (B) recruitment and number and Nfkb activation (C) were analyzed at 6, 12, and 24 hpi by fluorescence microscopy; the transcript levels of the indicated genes were analyzed at 12 hpi by RT-qPCR (D, F, and G); and caspase-1 activity was determined at 24 hpi using a fluorogenic substrate (E). Each dot represents one individual and the means ± SEM for each group is also shown. RT-qPCR data are depicted as a heatmap in (D) with higher expression shown in darker color. P values were calculated using one-way ANOVA and Tukey multiple range test. *P ≤ 0.05, **P ≤ 0.01, and ***P ≤ 0.001.

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

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