FIGURE

Fig. 2

ID

ZDB-FIG-220920-59

Publication

Tyrkalska et al., 2022 - Differential proinflammatory activities of Spike proteins of SARS-CoV-2 variants of concern

Other Figures

All Figure Page

Back to All Figure Page

Fig. 2

Fig. 2. WT S1 signals through the canonical inflammasome in zebrafish.

Recombinant S1WT or vehicle (−) were injected in the hindbrain ventricle (arrowheads) of 2-dpf Tg(mpx:eGFP) (A) and WT (B and C) larvae in the presence of either dimethyl sulfoxide (DMSO) or the caspase-1 inhibitor VX-765 (VX). Neutrophil recruitment and number were analyzed at 3 hpi by fluorescence microscopy (A), the transcript levels of the indicated genes were analyzed at 12 hpi by RT-qPCR (B), and caspase-1 activity was determined at 24 hpi using a fluorogenic substrate (C). Representative images of whole Tg(mpx:eGFP) larvae for each treatment are also shown (A). Each dot represents one individual and the means ± SEM for each group is also shown. RT-qPCR data are depicted as a heatmap in (B) with higher expression shown in darker color. P values were calculated using one-way ANOVA and Tukey multiple range test. *P ≤ 0.05, **P ≤ 0.01, and ***P ≤ 0.001. Scale bar, 500 μm.

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Sci Adv