Fig. 2
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- ZDB-FIG-220916-7
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- Mendieta-Serrano et al., 2022 - Slow muscles guide fast myocyte fusion to ensure robust myotome formation despite the high spatiotemporal stochasticity of fusion events
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Figure 2. Cell shape prior to fusion is highly heterogeneous (A) Top: example of images of cell shape from segmentation to fusion. Bottom: morphospace of fusing cells, defined with respect to the time from somite segmentation (t = 0 min). Individual dots represent the onset of fusion. Aspect ratio (Aiii) defined as the ratio of long to short axes of the best fitting ellipse to the cell cross-sectional shape. Scale bar, 10 μm. (B) Morphospace of fusing cells, defined with respect to their time before fusion (t = 0 represents the time when each cell fused). Shaded regions indicate 95% CI. (C) Relative morphospace of fusing cells, where the larger value of the two cells is in the numerator at each time point (hence, relative value is always greater than 1). Shaded regions indicate 95% CI (STAR Methods). Time defined as in (B). (A–C) 60 cells, from 4 segments from 2 embryos. (D) Example of fusing cell pairs, highlighting the cell perimeter, with color representing time. The rounder cell is positioned on top. Time defined as in (B). Scale bar, 10 μm. (E) Boundary capture analysis at fusion. 45 fusion events, from 3 myotome segments from 1 embryo. Scale bar, 10 μm. (F) Image of nocodazole-treated embryo (right, control on left) injected with lyn-gfp and h2b-mcherry mRNA. Left: scale bars, 50 μm. Right shows a close-up view of selected fused cells. Scale bars, 10 μm. Fusion events highlighted by white arrowheads. Time defined as in (B). |
Reprinted from Developmental Cell, 57(17), Mendieta-Serrano, M.A., Dhar, S., Ng, B.H., Narayanan, R., Lee, J.J.Y., Ong, H.T., Toh, P.J.Y., Röllin, A., Roy, S., Saunders, T.E., Slow muscles guide fast myocyte fusion to ensure robust myotome formation despite the high spatiotemporal stochasticity of fusion events, 2095-2110.e5, Copyright (2022) with permission from Elsevier. Full text @ Dev. Cell