FIGURE

Fig. 8

ID
ZDB-FIG-220914-91
Publication
Ratanayotha et al., 2022 - Voltage-sensing phosphatase (Vsp) regulates endocytosis-dependent nutrient absorption in chordate enterocytes
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Fig. 8

Expression profile of Ci-Vsp and Ci-Dab2 in ascidian C. intestinalis.

ac Expression profile of Ci-Vsp by WISH. a Whole-mount (left) 2-week-old juvenile showing regional expressions in the stomach (st; arrow), bulged-region of the mid-intestine (int; arrowhead), and posterior-intestine (gray arrowhead). Higher magnification (right) reveals a stripe-like distribution of signals in the stomach and bulged intestine. b Dissected post-pharyngeal region of the digestive tract of the 1.5-month-old young adult showing an additional expression region (white arrowhead) in the looped-intestine. c Transverse section specimens of the juvenile (white line in a) showing Ci-Vsp expressions in the epithelium of the digestive tract (left). Expression signals in the stomach epithelium are restricted mainly in the inner-folds (white dotted lines), which contain several absorptive enterocytes38, as opposed to the outer-folds (dotted lines) with many pancreatic-related exocrine gene expressions39. Circumferences of the stomach (middle) and bulged intestine (right) show alternating epithelial morphology and Ci-Vsp expression. df Expression profile Ci-Dab2. d Whole-mount (left) 2-week-old juvenile showing regional expressions in the stomach (st; arrow), bulged-region of the mid-intestine (int; arrowhead), and posterior-intestine (gray arrowhead). Higher magnification (right) reveals a stripe-like distribution of signals in the stomach and bulged intestine. e Dissected post-pharyngeal region of the digestive tract of the 1.5-month-old young adult showing an additional expression region (white arrowhead) in the looped-intestine. f Transverse section specimens of the juvenile (white line in d) showing Ci-Dab2 expressions in the epithelium of the digestive tract (left). Expression signals in the stomach epithelium are restricted mainly in the inner-folds (white dotted lines) as opposed to the outer-folds (dotted lines). Circumferences of the stomach (middle) and bulged intestine (right) show alternating epithelial morphology and Ci-Dab2 expression. Scale bars = 200 µm in a, d, 1 mm in b, e, and 50 µm in c, f. OS oral siphon, AS atrial siphon, Ph pharynx, En endostyle, Stg stigma, Es esophagus, Nc neural complex, Gd gonad.

Expression Data

Expression Detail
Antibody Labeling
Phenotype Data

Phenotype Detail
Acknowledgments
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