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FIGURE 1

ID

ZDB-FIG-220914-51

Publication

Hu et al., 2022 - Iron supplementation inhibits hypoxia-induced mitochondrial damage and protects zebrafish liver cells from death

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FIGURE 1

Hypoxia stress affects cell growth, ROS production and iron metabolism in cytoplasma and mitochondria. (A) Microscopic analysis of morphological changes of ZFL cells under normoxia or hypoxia for 1, 2, 3 and 4 d. (B) Cell Viability analyzed with PrestoBlue™ HS Cell Viability Regent under normoxia or hypoxia for 1, 2, 3 and 4 d. (C–E) Analysis of changes in total ROS (C), mitochondrial-derived ROS (D) and lipid peroxidation (E) levels in cells with CM-H2DCFDA, MitoSOX and C11-BODIPY probe under normoxia and hypoxia for 3 days. (F) Western blot analysis of Ferritin expression in ZFL cells cultured under normoxia and hypoxia for 3 days. (G) Phen Green™ SK (PGSK) probe analysis and quantification of cytoplasmic free iron content in ZFL cells after treated under normoxia and hypoxia for 3 days. (H) The mRNA expression of mitochondrial iron storage gene fth31 in ZFL cells quantified by real-time RT–PCR under normoxia and hypoxia for 3 days. (I) Fluorescence microscope with RPA red indicator analysis and quantification of mitochondrial iron content in ZFL cells treated under normoxia and hypoxia for 3 days. Normoxia was used as a control group for significance analysis. Error bars, mean ± s.d., n = 3 (biological replicates).

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Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Front. Physiol.