Figure 4

Zebrafish infection model with EmbA/GlfT2_KD strains. (A, B) Survival curves of larvae zebrafish infected by control PLJR962 strain and EmbA/GlfT2_KD strains with initial colony forming unit (CFU) = 100 or 300, respectively (n = 20–50). Log-rank tests are used to test the significance of the Kaplan–Meier survival curves: *P < 0.05, **P < 0.01. (C, D) CFU counts and the granuloma numbers in adult zebrafish infected by control strain and EmbA/GlfT2 knock-down strains, respectively, at 2 weeks post-injection. Data were plotted as mean ± SD. Two-way analysis of variance tests were used for all significance analysis: *P < 0.05 and **P < 0.01. (E) Acid-fast staining and hematoxylin and eosin (H&E) staining of zebrafish sections. Acid-fast staining was observed with an optical microscope at ×100 and ×200, and the visual field of H&E stain was the corresponding area of acid-fast staining at ×200 magnification. The red arrow points to the granuloma, and the purplish red color in the center of acid-fast granuloma represents the thallus.