Figure 3

EmbA/GlfT2_KD strain-infected murine macrophage J774A.1 line. (A, B) Macrophages were infected with a multiplicity of infection (MOI) = 1 at 48-h timepoints, and cell survival rates were determined using the cell counting kit (CCK8) at 24/48 h. The cell survival rate was calculated as follows: survival rate = [(As - Ab)/(Ac - Ab)] × 100%, in which As is the absorbance of the infected well of the control of knock-down groups, Ab is the absorbance of medium-only well without added cells, and Ac is the absorbance of uninfected cell wells. (C, D) The macrophages were infected with a MOI = 5 at 48-h timepoints, and the cell survival rate was determined by CCK8 at 24/48 h. (A–D) Data processing is homogenized, and data are plotted as mean ± SD. Two-way ANOVA tests are used for all significance analysis here: **P < 0.01, **P < 0.01, ***P < 0.001, and ****P < 0.0001.