FIGURE

Fig. 6

ID

ZDB-FIG-220912-166

Publication

Zhuang et al., 2022 - Histone Deacetylases Cooperate with NF-κB to Support the Immediate Migratory Response after Zebrafish Pronephros Injury

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Fig. 6

Histone deacetylases and NF-κB signaling cooperate to promote repair through migration. (A) Whole-mount in situ hybridization reveals that nfkbiaa is upregulated in the cells flanking the injury site and the expression is persistent in the first 4 h post injury (hpi). (B) An example of nfkbiaa SBM-injected embryo 24 h post ablation. The white arrow points to the injury site. (C) Depletion of nfkbiaa with splice blocking MO (SBM) significantly reduced the number of successful repairs. (D) nfkbiaa TBM-injected embryos repair at significantly lower rate than controls. (E) Combined depletion of hdac6 TBM, hdac8 TBM, and nfkbiaa TBM with low MO concentrations synergistically reduces the number of embryos that successfully repair the pronephros. The number of examined embryos is shown in brackets. The points represent individual experiments. Mean and standard deviation for each group are displayed. Significance was calculated with Fisher’s exact test (two-tailed).

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	li1Tg; uf1Tg + MO1-nfkbiaa li1Tg; uf1Tg + MO2-nfkbiaa li1Tg; uf1Tg + MO1-hdac6 + MO1-hdac8 + MO1-nfkbiaa
Condition:	light ablation: pronephros
Knockdown Reagents:	MO1-hdac6 MO1-hdac8 MO1-nfkbiaa MO2-nfkbiaa
Observed In:	pronephros wound healing
Stage:	Protruding-mouth

Phenotype Detail

Acknowledgments

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