FIGURE

Fig. 2

ID
ZDB-FIG-220905-2
Publication
Neuffer et al., 2022 - AP-3 complex delta subunit gene, ap3d1, regulates melanogenesis and melanophore survival via autophagy in zebrafish (Danio rerio)
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Fig. 2

crasher mutant phenotype recapitulation using CRISPR knockout. (a) The crasher mutation maps to a ~ 375 kb region on chromosome 22. R = recombination event. (b–g) CRISPR ap3d1 knockout in *AB lines recapitulates the crasher phenotype. (b) Uninjected *AB control. (c) tyr gRNA injected *AB fish have mosaic tyrosinase activity. (d) crasher mutant with slight lightening of the retinal pigmented epithelium. (e) ap3d1 gRNA injected *AB fish display light, punctate crasher-like melanophores and a lighter retinal pigmented epithelium than crasher mutant. (f) Melanophore count boxplot in uninjected and injected tyr and ap3d1 fish. Melanophore number is reduced in both populations injected with gRNA (two-way ANOVA, guide F(1, 56) = 5.50, p = <0.02; injection status F(1, 56) = 247.50, p = <0.22 × 10−15; interaction F(1, 56) = 23.68, p = <9.68 × 10−6, Tukey HSD performed to determine group differences, p = <0.001***). (g) Boxplot shows iridophore number is unchanged between uninjected and injected for both guides (two-way ANOVA, injection status F(1, 56) = 0.02, p = 0.88; guide F(1, 56) = 46.05, p = 7.80 × 10−9; interaction F(1, 56) = 0.89, p = 0.35, Tukey HSD performed to determine group differences, p = <0.001***). Scale bar is 500 μm. 78 eggs injected for tyr; 58 eggs injected for ap3d1.

Expression Data

Expression Detail
Antibody Labeling
Phenotype Data
Fish:
Knockdown Reagents:
Observed In:
Stage: Protruding-mouth

Phenotype Detail
Acknowledgments
This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Pigment Cell Melanoma Res.