FIGURE

Fig. 8

ID
ZDB-FIG-220823-8
Publication
Song et al., 2022 - GATA4/5/6 family transcription factors are conserved determinants of cardiac versus pharyngeal mesoderm fate
Other Figures
All Figure Page
Back to All Figure Page
Fig. 8

Gata4/5/6 is required to promote a cardiac and antagonize a pharyngeal muscle fate in Ciona.

(A) Summarized Ciona cardiopharyngeal lineage and expression of markers. Rounded squares, cells; arrows, cell divisions. Note the transient down-regulation of Gata4/5/6 and subsequent reactivation in FHP and SHP (first and second heart progenitors). (B) Schematic representation of cardiopharyngeal lineages at stages 24, 26, and 30. ASM ring (arrows); ATM, anterior tail muscle; black bars link sister cells. Endogenous expression of Tbx1/10 at stage 24 (C) and Ebf at stage 26 (E) visualized by ISH (green) in control (TyrosinaseCRISPR) and Gata4/5/6CRISPR knockout embryos. Proportions of embryos expressing Tbx1/10 (D) and Ebf (F) transcripts in indicated cell-type progenitors. (D) STVC only (Tyr: 97%, n = 48; Gata4/5/6CRISPR: 66%, n = 31), both STVC and FHP (Tyr: 3%, n = 3; Gata4/5/6CRISPR: 34%, n = 16). (F) ASMF only (Tyr: 100%, n = 70), both ASMF and SHP-FHP (Tyr: 0%, Gata4/5/6CRISPR: 43% ± 3% SE; n = 65). Nuclei, red; cell membranes, blue. (G) Target overexpression of Gata4/5/6 in cardiopharyngeal progenitor cells. B7.5 lineage cells, red. ASMP, atrial siphon muscle precursor cells. (H) Proportions of embryos with normal or reduced numbers of Ebf-expressing ASMPs. Control: 20% (n = 98), Gata4/5/6OE: 68 ± 3% SE (n = 118) reduced Ebf-expressing ASMPs. Fisher’s exact test was used. White asterisks, central nervous system (CNS). Scale bars, 10 or 15 μm (zoomed-in views).

Expression Data

Expression Detail
Antibody Labeling
Phenotype Data

Phenotype Detail
Acknowledgments
This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Sci Adv