FIGURE

Figure 5

ID

ZDB-FIG-220801-20

Publication

Gessler et al., 2022 - Control of Directed Cell Migration after Tubular Cell Injury by Nucleotide Signaling

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Figure 5

Effect of the combined adenosine pathway knockdown, and of the P2YR2 agonist Diquafosol on the repair process. (A) Combined depletion of ada2b, adora1b, p2ry2.1, p2ry2.2 and p2ry2.3 with low concentrations of MOs significantly delayed the repair process (mean ± SEM; 2-way Anova). (B) Diquafosol (1 mg/mL) did not affect zebrafish development. (C) Diquafosol exposure for 3 h before, and for 24 h after laser-induced injury resulted in accelerated repair (mean ± SEM; 2-way Anova). (D) Depletion of zebrafish p2ry2 variants by translation-blocking morpholino oligonucleotides (MO) prevented the accelerating effect of Diquafosol.

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	WT WT + MO1-p2ry2.1 + MO1-p2ry2.2 + MO1-p2ry2.3 WT + MO1-ada2b + MO1-adora1b + MO1-p2ry2.1 + MO1-p2ry2.2 + MO1-p2ry2.3
Conditions:	ablation: pronephros, chemical treatment by environment: P(1),P(4)-bis(uridin-5'-yl) tetraphosphate ablation: pronephros
Knockdown Reagents:	MO1-ada2b MO1-adora1b MO1-p2ry2.1 MO1-p2ry2.2 MO1-p2ry2.3
Observed In:	pronephros pronephros regeneration
Stage:	Long-pec

Phenotype Detail

Acknowledgments

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