Figure 3

Tumorigenic potential of a lung carcinoid PDX in zebrafish embryos. Red-stained cells, obtained from a patient surgical resection, were used to perform PDX in 48 hpf Tg(fli1a:EGFP)^y1 zebrafish embryos. Epifluorescence images at 24 hpi of cell resuspension solution injected embryos (control: a) and patient-derived lung carcinoid (red) xenografted embryos (b,c). The red channel was omitted in panels (b,b′) to facilitate the observation of tumor-induced angiogenesis (green); b′ is the digital magnification of the white-boxed region. Lung carcinoid PDX induced the formation of endothelial structures (green) sprouting from the SIV within 24 hpi. Overlay of representative fluorescent and bright field images of grafted embryos at 0 (d) and 48 hpi (e,f) showed the spread of tumor cells throughout the embryo body. The tail, particularly at 48 hpi, was imaged (f). Embryos are shown anterior to the left. Scale bar: 100 µm.