Fig. 3

(A) Lateral view images after staining with anti-SV2 and α-BTX of the whole-mounted zebrafish injected with control, gars MOs, and human WT mRNA at 84 hpf. The merged images (NMJs) are magnified from the images in the rectangular regions. Scale bars = 50 μm. (B) Comparisons of fluorescence intensity (green, presynapse; red, postsynapse) among MOs and mRNAs injected zebrafish embryos. (C-E) Comparison of presynapse (C), postsynapse (D), and NMJ (E) signal ratios within the ROI among zebrafish embryos injected with control MOs (n = 22), gars MOs (n = 22), and gars MOs with WT (n = 33) mRNAs. Statistical significance was assessed using one-way ANOVA followed by Tukey’s post hoc test. ***P < 0.001. ns, non-significant. (F and G) Comparison of NMJ innervation in the presynaptic area (F) and postsynaptic area (G) among zebrafish embryos injected with control MOs (n = 15), gars MOs (n = 19), and gars MOs with human WT GARS mRNAs (n = 30). Statistical significance was determined using one-way ANOVA followed by Tukey’s post hoc test. ***P < 0.001. ns, non-significant.